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EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine.

Plant Cell Rep.. 2013-12; 
Y Acanda, ? MartÍnez, MJ Prado, MV GonzÁlez, M Rey. Departamento de BiologÍa Vegetal y Ciencia del Suelo, Universidad de Vigo, Campus Universitario, 36310, Vigo, Spain.
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摘要

Embryogenic suspension cultures are suitable for EMS mutagenesis in grapevine, and HRM prescreening of EMS-treated somatic embryo clusters allows rapid detection of point mutations before plant regeneration. Somatic embryogenesis is an excellent system for induced mutagenesis and clonal propagation in woody plants. Our work was focused on establishing a procedure for inducing ethyl methanesulfonate (EMS) mutagenesis in grapevine. Embryogenic cell aggregates (ECAs) growing in liquid medium were treated with increasing concentrations of EMS. We found that EMS dramatically affects the viability of ECAs at concentrations above 20 mM (25.5 ± 2.9 % survival), whereas concentrations above 10 mM affect embryogen... More

关键词

Grapevine; Ethyl methanesulfonate; Embryogenic suspension; NCED; qPCR-HRM
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