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Impact of Allogrooming in Domestic Cats (Felis silvestris catus) on Mitochondrial DNA Profiling of Shed Hairs.

Open Forensic Sci J.. 2013-11; 
Robert A. Grahn, Tina I-T Huang and Leslie A. Lyons. Department of Population Health & Reproduction, School of Veterinary Medicine, University of California, Davis,Davis, CA 95616, USA
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摘要

Allogrooming is a normal behavior for socially bonded cats. Cat hairs may have epithelial cells on the shaft from at least two cat contributors, the host (groomee) and the donor (groomer). To determine the likelihood of obtaining a mixture or incorrect DNA profile in cat hairs, feline mtDNA control region from hairs of allogrooming cats was isolated and analyzed by direct sequencing. Two DNA extraction methods were tested; hair washes and complete digestion of hairs. For five allogrooming pairs with different mitotypes, thirteen of the 126 sequences (10.3%) matched the mitotype of the groomer, not the groomee. Forty-three sequences (34.13%) suggested the presence of both mitotypes, groomer and groomee. Approxim... More

关键词

Forensic science; control region; heteroplasmy; feline; mitochondrial DNA; mitotype
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