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Recombinantly expressed virus-like particles (VLPs) of canine circovirus for development of an indirect ELISA

Vet Res Commun. 2024-01; 
Alison Neef, Babu Kanti Nath, Tridip Das, Daniel Luque, Jade K Forwood, Shane R Raidal, Shubhagata Das
Products/Services Used Details Operation
Gene Synthesis … In this study, full length CanineCV capsid gene was expressed in Escherichia coli followed by … gene synthesis was conducted by GenScript Biotech (Singapore). Then the target genes … Get A Quote

摘要

Canine circovirus (CanineCV) is an emerging pathogen in domestic dogs, detected in multiple countries in association with varying clinical and pathological presentations including diarrhoea, vasculitis, granulomatous inflammation, and respiratory signs. Understanding the pathology of CanineCV is confounded by the fact that it has been detected in asymptomatic dogs as well as in diseased dogs concurrently infected with known pathogens. Recombinantly expressed self-assembling Virus-like particles (VLPs) lack viral genomic material but imitate the capsid surface conformations of wild type virion, allowing arrays of biological applications including subunit vaccine development and immunodiagnostics. In this study, ... More

关键词

Canine circovirus, Indirect ELISA, Recombinant expression, Virus like particles
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