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The HIV-1 capsid serves as a nanoscale reaction vessel for reverse transcription

biorxiv. 2023-11; 
Jordan Jennings, Harrison Bracey, Danny T Nguyen, Rishav Dasgupta, Alondra Vázquez Rivera, Nicolas Sluis-Cremer, Jiong Shi, Christopher Aiken
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Proteins, Expression, Isolation and Analysis … Env proteins from other viruses can support NERT. Pseudotyped HIV-1 particles bearing the Env proteins of … on precast 4-20% gradient gels using Tris-MOPS running buffer (Genscript). … Get A Quote

摘要

The viral capsid performs critical functions during HIV-1 infection and is a validated target for antiviral therapy. Previous studies have established that the proper structure and stability of the capsid are required for efficient HIV-1 reverse transcription in target cells. Moreover, it has recently been demonstrated that permeabilized virions and purified HIV-1 cores undergo efficient reverse transcription in vitro when the capsid is stabilized by addition of the host cell metabolite inositol hexakisphosphate (IP6). However, the molecular mechanism by which the capsid promotes reverse transcription is undefined. Here we show that wild type HIV-1 particles can undergo efficient reverse transcription in the a... More

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