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Studies on short tandem repeat genotyping and its expert system based on ultraviolet spectroscopy-principal discriminant variate.

Chemometr Intell Lab Syst.. 2011-02;  105(2):181-187
Z Wu, H Lu, B Zhang, L Xie, Y Gao, H Xie, Bingren Gu. College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, PR China.
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摘要

The paper has presented a method which could be used to detect short tandem repeat (STR) rapidly, simply and low-costly based on ultraviolet spectroscopy combined with principal discriminant variate (PDV) of chemical pattern recognition. Genotypes 10-11, 11-11 and 10-12 of STR locus D16S539 which was commonly used in forensic medicine were used to establish two-classified PDV genotyping models. The conditions of polymerase chain reaction (PCR) and ultraviolet spectral detection were optimized. Based on the optimal PCR conditions, each genotype was amplified twice in parallel. For the PDV model of 10-11 and 11-11, the prediction samples were all distributed in the scope of calibration samples, the between-class ... More

关键词

Short tandem repeat; Ultraviolet spectroscopy; Principal discriminant variate; Genotyping; Expert system
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