至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Novel method for high-throughput colony PCR screening in nanoliter-reactors.

Nucleic Acids Res.. 2009-05; 
Walser Marcel, Pellaux Rene, Meyer Andreas, Bechtold Matthias, Vanderschuren Herve, Reinhardt Richard, Magyar Joseph, Panke Sven, and Held Martin. ETH Zurich, Institute of Process Engineering, BioProcess Laboratory (BPL), Zurich, Switzerland.
Products/Services Used Details Operation

摘要

We introduce a technology for the rapid identification and sequencing of conserved DNA elements employing a novel suspension array based on nanoliter (nl)-reactors made from alginate. The reactors have a volume of 35 nl and serve as reaction compartments during monoseptic growth of microbial library clones, colony lysis, thermocycling and screening for sequence motifs via semi-quantitative fluorescence analyses. nl-Reactors were kept in suspension during all high-throughput steps which allowed performing the protocol in a highly space-effective fashion and at negligible expenses of consumables and reagents. As a first application, 11 high-quality microsatellites for polymorphism studies in cassava were isolated... More

关键词

Baidu
map