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Single-feature polymorphism discovery in the transcriptome of tetraploid alfalfa.

Plant Genome.. 2009-11;  2:224-32
S. Samuel Yang, Wayne Wenzhong Xu, Mesfin Tesfaye, JoAnn F. S. Lamb, Hans-Joachim G. Jung, Deborah A. Samac, Carroll P. Vance, John W. Gronwald. Supercomputing Institute for Advanced Computational, Research, Univ. of Minnesota, 117 Pleasant St. SE, Minneapolis, MN 55455
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摘要

Advances in alfalfa [Medicago sativa (L.) subsp. sativa] breeding, molecular genetics, and genomics have been slow because this crop is an allogamous autotetraploid (2n = 4x = 32) with complex polysomic inheritance and few genomic resources. Increasing cellulose and decreasing lignin in alfalfa stem cell walls would improve this crop as a cellulosic ethanol feedstock. We conducted genome-wide analysis of single-feature polymorphisms (SFPs) of two alfalfa genotypes (252, 1283) that differ in stem cell wall lignin and cellulose concentrations. SFP analysis was conducted using the Medicago GeneChip (Affymetrix, Santa Clara, CA) as a cross-species platform. Analysis of GeneChip expression data files of alfalfa stem... More

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