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Detection of intestinal pathogenic Klebsiella pneumoniae from fecal samples of Giant Panda by Polymerase Chain Reaction.

J Biotech Res.. 2013-07; 
F Sun, S Yang, X Chen. School of Environmental and Chemical Engineering, Xi'an Polytechnic University, No. 19 Jinhua South Road, Xi'an 710048, China
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摘要

It was necessary to develop simple and reproducible diagnostic tools for endangered species, and their associated pathogens. Klebsiella pneumoniae was the main pathogenic bacterium leading to intestinal disease within Giant Panda (Ailuropoda melanoleuca). A noninvasive method for the specific detection of K. pneumoniae in Giant Pandas' s feces was developed and evaluated in this study. The 368-bp phoE gene was selected for specificity, and 2 types of K. pneumoniae strains in conjunction with 16 non-K. pneumoniae strains were evaluated. The Key step of noninvasive sampling procedure was to remove the inhibitors of Polymerase Chain Reaction (PCR), which was based on that silica powders could bind pathogen DN... More

关键词

PCR; Ailuropoda melanoleuca; noninvasive technique; diagnosis
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