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Hydroxyapatite Nanoparticle-Crosslinked Peptide Hydrogels for Three-Dimensional Culture and Differentiation of MC3T3-E1 Osteoblasts.

J Biomed Nanotechnol. 2019; 
Xu Y, Wu X, Wang S, Yang C, Li Y, Cao Y.
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Proteins, Expression, Isolation and Analysis … Cell lysis buffer (cat: P0013, Beyotime, China); a Pierce™ BCA Protein Assay Kit (cat:23227, Thermo Scientific™, USA) for total pro- tein quantification, Tris-MOPS-SDS electrophoresis run- ning buffer (cat: M00138, GenScript, China); SurePAGE™ precast mini polyacrylamide … Get A Quote

摘要

Hydrogels have been widely used to mimic the biochemical and mechanical environments of native extracellular matrices for cell culture and tissue engineering. Among them, self-assembling peptide hydrogels are of special interest thanks to their great biocompatibility, designability and convenient preparation procedures. In pioneering studies, self-assembling peptide hydrogels have been used for the culture of bone marrow cells. However, the low mechanical stability of peptide hydrogels seems to be a drawback for these applications, as bone marrow cells prefer hard substrates for osteogenic differentiation. In this work, we explored the use of hydroxyapatite (HAP)-peptide hybrid hydrogels for three-dimensional (... More

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