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Early detection of aquatic invaders using metabarcoding reveals a high number of non‐indigenous species in Canadian ports.

Diversity and Distributions.. 2016-10; 
Emily A. Brown,Frédéric J. J. Chain,Aibin Zhan,Hugh J. MacIsaac,Melania E. Cristescu.
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Molecular Biology Reagents ... 2014). PCR mixtures (25 μL) contained approximately 100 ng of genomic DNA, 1 × PCR buffer, 2 mm of Mg 2+ , 0.2 mm of dNTPs, 0.4 μm of each primer and 2 U of Taq DNA polymerase (Genscript, Piscataway, NJ, USA). PCR ... Get A Quote

摘要

Aim Invasive species represent one of the greatest threats to biodiversity. The ability to detect non-indigenous species (NIS), particularly those present at low abundance, is limited by difficulties in performing exhaustive sampling and in identifying species. Here we sample zooplankton from 16 major Canadian ports and apply a metabarcoding approach to detect NIS. Location Marine and freshwater ports along Canadian coastlines (Pacific, Arctic, Atlantic) and the Great Lakes. Methods We amplified the V4 region of the small subunit ribosomal DNA (18S) and used two distinct analytic protocols to identify species present at low abundance. Taxonomic assignment was conducted using BLAST searches against a local 18... More

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