We present a method to monitor in real time peptide self-assembly or polymerization events. The temperature controlled modification of a previously reported splash test setup using high speed imaging enables to observe and measure rheological changes in liquid samples and can, in turn, monitor a peptide self-assembly or polymerization reaction accompanied with specific changes in solution viscosity. A series of 2 mm glass beads were dropped into an Fmoc-L3-OMe (methylated Fluorenylmethyloxycarbonyl-trileucine) solution mixed with Alcalase 2.4 L (EC 3.4.21.62) or first dipped in Tetramethylethylenediamine (TEMED), a catalyst for acrylamide polymerization, then dropped into acrylamide. The resulting splashes were... More
We present a method to monitor in real time peptide self-assembly or polymerization events. The temperature controlled modification of a previously reported splash test setup using high speed imaging enables to observe and measure rheological changes in liquid samples and can, in turn, monitor a peptide self-assembly or polymerization reaction accompanied with specific changes in solution viscosity. A series of 2 mm glass beads were dropped into an Fmoc-L3-OMe (methylated Fluorenylmethyloxycarbonyl-trileucine) solution mixed with Alcalase 2.4 L (EC 3.4.21.62) or first dipped in Tetramethylethylenediamine (TEMED), a catalyst for acrylamide polymerization, then dropped into acrylamide. The resulting splashes were observed using a high speed camera. The results demonstrate that the viscosity changes of the peptide sample during the peptide self-assembly or acrylamide polymerization affect the specific shape and evolution of the splashing event. Typically, the increase in viscosity while the reaction occurs decreased the size of the splash and the amount of time for the splash to reach maximum extension from the moment for the beads to impact the sample. The ability to observe rheological changes of sample state presents the opportunity to monitor the real time dynamics of peptide self-assembly or cross-polymerization.
Real-time monitoring of viscosity changes triggered by chemical reactions using a high-speed imaging method (PDF Download Available). Available from: https://www.researchgate.net/publication/277338392_Real-time_monitoring_of_viscosity_changes_triggered_by_chemical_reactions_using_a_high-speed_imaging_method [accessed Nov 08 2017].
