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Characterization of Arginine Kinase in the Barnacle Amphibalanus Amphitrite and Its Role in the Larval Settlement.

J Exp Zool B Mol Dev Evol.. 2016-06; 
Zhang G,Yan GY,Yang XX,Wong YH,Sun J,Zhang Y,He LS,Xu Y,Qian PY.
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Proteins, Expression, Isolation and Analysis ... washes with the IP buffer. At last, the beads were boiled at 100°C for 10 min in 1× Laemmli sample loading buffer and the eluted proteins were visualized on a 12% SDS-PAGE gel (Genscript, Nanjing, China). A control was carried ... Get A Quote

摘要

Energy metabolism is a key process in larval settlement of barnacles, but the underlying molecular mechanisms remain ambiguous. Arginine kinase (AK) mainly participates in energy metabolism in invertebrates. So far, its roles in barnacles have not been studied. In the present study, we raised an antibody against AK from Amphibalanus amphitrite Darwin to characterize the roles of AK in the larval settlement process. Among the developmental stages, AK was highly expressed during the cypris stage. Along with the aging process in cyprids, the level of AK decreased. The immunostaining results showed that AK was localized to muscular tissues in cyprids, including antennules, antennular muscles, and thoracic limbs. Th... More

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