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Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR.

J Virol Methods.. 2016-09; 
Thekke Veetil T,Ho T,Moyer C,Whitaker VM,Tzanetakis IE.
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Gene Synthesis ... The reaction mixture (25 μl) contained equal amount of virus and internal control primers (400 nM each), 10X Taq buffer (proprietary), 400 μm dNTPs, 0.5U of Taq DNA polymerase (Genscript, NJ) and RNAse/DNase-free water. ... Get A Quote

摘要

Graft-indexing of an advanced selection from the University of Florida strawberry breeding program produced virus-like symptoms on Fragaria vesca. However; RT-PCR testing of the material did not detect the presence of any of 16 strawberry virus species or members of virus groups for which strawberries are routinely indexed. Large scale sequencing of the material revealed the presence of an isolate of Strawberry necrotic shock virus. The nucleotide sequence of this isolate from Florida shows a significant number of base changes in the annealing sites of the primers compared to the primers currently in use for the detection of SNSV thereby explaining the most probable reason for the inability to detect the virus ... More

关键词

Strawberry necrotic shock virus; Taqman qPCR; Virus detection
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