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Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease.

PLoS One.. 2016-08; 
Santos FL, Celedon PA, Zanchin NI, Brasil Tde A, Foti L, Souza WV, Silva ED, Gomes Yde M, Krieger MA.
Products/Services Used Details Operation
Bacterial Protein Expression System ... purification. Optimized synthetic genes for E. coli expression of the four T. cruzi chimeric antigens (named IBMP8-1, IBMP8-2, IBMP8-3 and IBMP8-4) were acquired from a commercial supplier (GenScript, Piscataway-NJ, USA). ... Get A Quote

摘要

The performance of serologic tests in chronic Chagas disease diagnosis largely depends on the type and quality of the antigen preparations that are used for detection of anti-Trypanosoma cruzi antibodies. Whole-cell T. cruzi extracts or recombinant proteins have shown variation in the performance and cross-reactivity. Synthetic chimeric proteins comprising fragments of repetitive amino acids of several different proteins have been shown to improve assay performances to detect Chagasic infections. Here, we describe the production of four chimeric T. cruzi proteins and the assessment of their performance for diagnostic purposes. Circular Dichroism spectra indicated the absence of well-defined secondary structures... More

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