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Characterization of the membrane-inserted C-terminus of cytoprotective BCL-XL.

Protein Expr Purif.. 2016-06; 
Yao Y, Nisan D, Fujimoto LM, Antignani A, Barnes A, Tjandra N, Youle RJ, Marassi FM.
Products/Services Used Details Operation
Peptide Synthesis ... Pure, chemically synthesized, 15 N Leu labeled BCL-XL(Ct) tail peptide was also obtained commercially (GenScript). NMR experiments. Samples for NMR studies were transferred to NMR buffer (20 mM Na-phosphate pH 6.5, 2 mM DTT, 1 mM EDTA). ... Get A Quote

摘要

BCL-XL is a dominant inhibitor of apoptosis and a significant anti-cancer drug target. Endogenous BCL-XL is integral to the mitochondrial outer membrane (MOM). BCL-XL reconstituted in detergent-free lipid bilayer nanodiscs is anchored to the nanodisc lipid bilayer membrane by tight association of its C-terminal tail, while the N-terminal head retains the canonical structure determined for water-soluble, tail-truncated BCL-XL, with the surface groove solvent-exposed and available for BH3 ligand binding. To better understand the conformation and dynamics of this key region of BCL-XL we have developed methods for isolating the membrane-embedded C-terminal tail from its N-terminal head and for preparing protein sui... More

关键词

Apoptosis; BCL-2; Membrane; NMR; Nanodisc; Structure
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