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Protein microarray-mediated detection of antienterovirus antibodies in serum.

J Int Med Res.. 2016-04; 
Zhang A, Xiu B, Zhang H, Li N.
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Proteins, Expression, Isolation and Analysis ... Following culture of transfected E. coli, His-tagged recombinant proteins were purified using Ni-NTA resin (Genscript, Hong Kong, China). The proteins were eluted with 6 M guanidine hydrochloride buffer (pH 7.9), then precipitated with trichloroacetic acid. ... Get A Quote

摘要

OBJECTIVE: To utilize prokaryotic gene expression and protein microarray to develop and evaluate a sensitive, accurate protein microarray assay for detecting antienterovirus antibodies in serum samples from patients with hand, foot and mouth disease (HFMD). Enterovirus 71 (EV71) and coxsackievirus A16 (CA16), two common causative agents for HFMD, were used for assay development. METHODS: Serum was collected from patients with HFMD and healthy controls. EV71 and CA16 VP1 and VP3 genes were expressed in transfected Escherichia coli; the resultant VP1 and 3 proteins were used in a microarray assay for human serum EV71 and CA16 immunoglobulin (Ig) M and IgG. To validate the microarray assay, serum samples were te... More

关键词

CA16; EV71; Hand; foot and mouth disease; protein microarray
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