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Characterization of Cry Proteins in Native Strains of Bacillus thuringiensis and Activity Against Anastrepha ludens 1.

Society of Southwestern Entomologists. 2015;  40(1):15-24
S. Buentello-Wong, L. GalÁn-Wong, K. Arévalo-Niño, V. Almaguer-CantÚ?and G. Rojas-Verde Instituto de BiotecnologÍa - Facultad de Ciencias BiolÓgicas - Universidad AutÓnoma de Nuevo LeÓn, Ave. Pedro de Alba s/n cruz con Ave. Manuel L. BarragÁn s/n,/ Cd. Universitaria, C.P. 66450 San NicolÁs de los Garza, Nuevo LeÓn, MÉxico
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摘要

Bacillus thuringiensis (Berliner) strains isolated from soil of citrus orchards were tested for insecticidal activity against the Mexican fruit fly, Anastrepha ludens (Loew), a key citrus pest in Mexico. From a total of 55 soil samples, 201 isolates were selected, for a total B. thuringiensis index of 0.66. The collection was characterized through light microscopy, polyacrylamide gel electrophoresis (SDS-PAGE), and PCR analysis detecting cry2, cry4, cry10, cry11, and cry19 genes. Of the 201 isolates, 51% produced ovoid crystals, 28% adhered to the spore, 15% were pleomorphic, 3% were bipyramidal, 2% cubic, and 1% was pyramidal type. Six colonies were positive for the cry10 gene and one for the cry19 gene. SDS-P... More

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