| Product Description |
CytoSinct CD8 Nanobeads, human is used forin vitroenrichment of CD8+cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension based on the surface expression of human CD8.
To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or other compatible columns with a magnet.
The CD8+cells labeled with Nanobeads are retained within the column and unlabelled cells (CD8-cells) are flushed out during the wash step. After removing the column from the magnetic field, the retained CD8+cells can be eluted as the positively selected cell fraction.
|
| Format |
Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188.
|
| Components |
1 mL or 0.5 mL CytoSinct CD8 Nanobeads, humanNanobeads conjugated to monoclonal anti-human CD8 antibodies (isotype: mouse IgG1).
|
| Volume |
| Cat. No. |
Size |
Capacity |
| L00864-0.5 |
500 µL |
Up to 5 × 108total cells |
| L00864-1 |
1 mL |
Up to 1 × 109total cells |
|
| Storage |
Store at 2 - 8 °C. Do not freeze.
|
CD8+T cells were enriched from human PBMCs. CD8+cells were stained with anti-CD8 (clone: SK1) BV650 and gated on Live/hCD45+.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
